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以高产优质橡胶品种热研88-13的幼嫩种子内珠被为外植体材料,对愈伤组织诱导培养基和继代培养基中的影响因素如植物生长调节剂的种类和浓度、钙离子浓度等进行了研究。经过连续6个月的继代选择培养,逐渐诱导出易碎的胚性愈伤组织,适宜的易碎胚性愈伤组织诱导培养基为:MH基本成分,添加0.5 mg/L KT,2.0 mg/L 2,4-D,11.0 mmol/L CaCl2,80.0 g/L蔗糖和2.0 g/L Phytagel。组织学切片证明长期继代培养的易碎胚性愈伤组织维持了胚性的状态。取继代培养2 a多的易碎胚性愈伤组织诱导体细胞胚的形成,得到了16 000多个体细胞胚。将这些胚进行进一步的转移培养,得到了115株再生植株。
With the high yield and high quality rubber variety Zheyan 88-13, the young seeds of Beidai seeds were used as explant materials. The influence factors of callus induction medium and subculture medium such as the type and concentration of plant growth regulator, Ion concentration and other research. The friable embryogenic callus was gradually induced after six consecutive months of subculture selection. The suitable embryogenic callus induction medium was: MH, 0.5 mg / L KT and 2.0 mg / L 2,4-D, 11.0 mmol / L CaCl2, 80.0 g / L sucrose and 2.0 g / L Phytagel. Histological sections demonstrated that long-term subculture of fragile embryogenic callus maintained embryogenic status. More than 2 a subcultures of embryogenic callus were selected to induce the formation of somatic embryos, and more than 16 000 somatic embryos were obtained. These embryos were further transferred and cultured, and 115 regenerated plants were obtained.