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目的:探讨骨髓间充质干细胞(bone marrowmesenchymal stem cells,BMSCs)与聚乳酸/羟基乙酸共聚物(poly lactide-co-glycolide,PLGA)三维生物支架在软骨源性形态发生蛋白1(cartilage-derived morphogenetic protein1,CDMP1)和转化生长因子-β1(transforming growth factor-β1,TGF-β1)作用下向软骨细胞表型分化及体内修复喉软骨缺损的能力。方法:在体外高密度细胞悬液与PLGA共同构筑的三维立体培养体系下CDMP1和(或)TGF-β1联合诱导BMSCs向软骨细胞分化,观察诱导后细胞表型的表达;将培养体系移植入动物体内,从大体、组织学方面观察其对喉软骨缺损的修复效果。结果:诱导后的培养体系可表达特异性软骨基质Ⅱ型胶原和GAG;将培养体系移植入动物体内,可有效地修复喉软骨缺损。结论:BMSCs与PLGA三维生物支架在CDMP1和TGF-β1作用下所得组织工程化软骨可以有效地修复喉软骨缺损。
OBJECTIVE: To investigate the expression of cartilage-derived morphogenetic protein 1 (BMSCs) and polylactide-co-glycolide (PLGA) protein1, CDMP1 and transforming growth factor-β1 (TGF-β1) to chondrocyte phenotypes and repair laryngeal cartilage defects in vivo. Methods: BMSCs were induced to differentiate into chondrocytes by CDMP1 and / or TGF-β1 in a three-dimensional culture system co-constructed with high-density cell suspension and PLGA in vitro. The expression of cell phenotype after induction was observed. The culture system was transplanted into animals In vivo, from the general, histological observation of its repair of laryngeal cartilage defects. Results: The induced culture system could express specific cartilage matrix type Ⅱ collagen and GAG. The culture system was transplanted into animals, which could effectively repair laryngeal cartilage defects. CONCLUSION: BMSCs and PLGA three-dimensional scaffolds can effectively repair laryngeal cartilage defects under the action of CDMP1 and TGF-β1.