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目的探讨过继转移血吸虫感染鼠树突状细胞(DC)对卵清蛋白(OVA)诱导的肺组织细胞CCL-11、IL-13Rα2表达和嗜酸性粒细胞浸润的影响。方法 20只BALB/c小鼠随机分成正常对照组(N组)、单纯哮喘组(OVA组)、诱发哮喘后过继转移血吸虫感染鼠DC组(OVA/SJDC组)和诱发哮喘后过继转移正常鼠DC组(OVA/NDC组)。各组最后一次鼻饲后24 h,处死小鼠,取左肺,切片,行HE染色和免疫组化染色,观察炎症变化,检测CCL-11和IL-13Rα2在肺组织中的表达。结果肺组织切片HE染色显示,OVA/SJDC组小鼠炎症较轻;OVA和OVA/NDC组炎症明显,有大量炎细胞浸润。免疫组化染色显示,OVA、OVA/SJDC和OVA/NDC小鼠肺组织CCL-11阳性区域平均吸光密度值分别为0.387 4±0.023 6、0.254 6±0.017 2和0.316 0±0.023 5,IL-13Rα2阳性区域平均光密度值分别为0.401 4±0.0130、0.280 8±0.010 2和0.326 6±0.014 4,差异均有统计学意义(P均<0.05)。结论日本血吸虫感染鼠DC能抑制OVA诱导的CCL-11和IL-13α2表达,从而对哮喘有治疗作用。
Objective To investigate the effects of dendritic cells (DCs) infected by adoptive transfer on the expression of CCL-11, IL-13Rα2 and eosinophil infiltration induced by ovalbumin (OVA) in lung tissue. Methods Twenty BALB / c mice were randomly divided into normal control group (N group), asthma group (OVA group), DC group (OVA / SJDC group) and adoptive transfer of normal mice DC group (OVA / NDC group). Twenty-four hours after the last nasal feeding, mice were sacrificed and the left lung was taken and sliced. The expression of CCL-11 and IL-13Rα2 in lung tissue were detected by HE staining and immunohistochemistry. Results HE staining showed that the mice in OVA / SJDC group had less inflammation. The inflammation in OVA and OVA / NDC group was obvious with a large number of inflammatory cell infiltration. Immunohistochemical staining showed that the average absorbance density of CCL-11 positive area of lung tissue of OVA, OVA / SJDC and OVA / NDC mice were 0.387 4 ± 0.023 6,0.254 6 ± 0.017 2 and 0.316 0 ± 0.023 5, IL- The average optical density values of 13Rα2 positive area were 0.401 4 ± 0.0130,0.280 8 ± 0.010 2 and 0.326 6 ± 0.014 4, respectively (all P <0.05). Conclusions DCs infected with Schistosoma japonicum can inhibit the expression of CCL-11 and IL-13α2 induced by OVA and thus have a therapeutic effect on asthma.