人尿液N-糖蛋白/N-糖肽规模化富集鉴定

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蛋白质的N-糖基化是真核细胞中一种重要的翻译后修饰,N-糖基化修饰在调控细胞黏附、迁移、信号转导及细胞凋亡等方面扮演着关键角色。蛋白质糖基化修饰的异常变化与多种重要疾病的发生相关。尿液具有蛋白质组复杂程度低和非入侵性等特点,适合大量及连续多时间点采样研究。但由于个体差异和生理条件的影响,尿蛋白丰度的生理波动较大。目前缺乏对健康人群尿液N-糖蛋白的个体差异和生理波动的专门性研究,以及生理丰度范围的构建,难以将个体差异、正常生理波动和疾病导致的变化进行有效区分,对疾病标志物研究提出很大挑战。本研究以亲水相互作
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