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Objective To compare the different time of administration of hCG affecting pregnancy rate of IUI.Methods A total of 189 infertile couples underwent 331 cycles of IUI with husband’s sperm.They were separated into 3 groups according to the time of hCG administration in IUI:hCG 1-23 h(group A):hCG 24-36 h (group B);hCG 37—48 h(group C).Results There were no statistical differences among 3 groups.None of the other relative factors,such as the female age,the different methods of ovulation and the cause of infertility,showed differences in pregnancy rate among 3 groups.Conclusion IUI can be performed any time after administration of hCG(1—48 h).