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目的观察胸导管淋巴引流在重症急性胰腺炎(SAP)诱发肝损伤的作用,为指导临床治疗提供实验依据。方法大鼠随机分为假手术组(S组)、重症胰腺炎模型组(SAP组)、胸导管引流组(TD组);每组又分为2、6、12h3个时间点。采用经胰胆管逆行注射5%牛磺胆酸钠法制备大鼠急性胰腺炎模型,检测大鼠腹水情况、血浆中肝功能指标(ALT、AST、TBIL)、TNF-α和IL-10的含量及胰腺和肝脏的病理变化。结果与SAP组比较,TD组腹水量明显减少,颜色变浅;ALT、AST、TBIL的含量明显下降(P<0.05或0.01);TNF-α和IL-10的含量明显下降(P<0.05或<0.01);显著改善胰腺和肝脏的病理损伤。结论胸导管引流可减轻重症急性胰腺炎合并的肝脏损伤。
Objective To observe the effect of thoracic duct lymphatic drainage on liver injury induced by severe acute pancreatitis (SAP) and provide experimental evidence for clinical treatment. Methods The rats were randomly divided into sham operation group (S group), severe pancreatitis model group (SAP group) and thoracic duct drainage group (TD group). Each group was divided into 3 groups at 2, 6 and 12 hours. Rat models of acute pancreatitis were prepared by retrograde injection of 5% sodium taurocholate via pancreaticobiliary duct. The ascites in rats, the levels of hepatic function (ALT, AST, TBIL), TNF-α and IL-10 in plasma And the pathological changes of pancreas and liver. Results Compared with SAP group, the amount of ascites in TD group was significantly reduced and the color was lighter; the levels of ALT, AST and TBIL were significantly decreased (P <0.05 or 0.01); the levels of TNF-α and IL-10 were significantly decreased <0.01); significantly improve the pathological injury of the pancreas and liver. Conclusion Drainage of thoracic duct can reduce liver injury associated with severe acute pancreatitis.