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本文评价了一种新的丙型肝炎病毒(HCV)基因分型法HCV Monitor Denotype~(TM) Test,该法系基于对HCV RNA定量检测(HCV Amplicor Monitor~(TM) Test)产物的分析。此项研究比较了新方法与DNA直接测序法的结果,以及未患血友病与血友病患者的HCV基因型和病毒载量。这种新的基因分型方法为:在HCV RNA定量检测法(Roche Diagnostic Systems)确定HCV的病毒载量后,将5′非翻译区(5′UTR)聚合酶链反应(PCR)产物(-274～-31核苷酸)纯化,进一步用一对套式PCR引物Geno-5(-27～-252核苷酸)和Geno-6(-64～-43核苷酸)扩增。套式PCR产物用Geno-7(-210～-189核苷酸)引物测序,只需分析5个核苷酸位点(-167、-163、-161、-160、-99核苷酸)就可区分6种基因型。研究选取了162个患慢性丙
This article evaluates a new hepatitis C virus HCV genotyping assay, HCV Monitor Denotype ™, based on the analysis of HCV RNA (HCV Amplicor Monitor ™) products. This study compared the results of the new method with DNA direct sequencing and HCV genotype and viral load in patients without hemophilia and hemophilia. The new genotyping method was to amplify the 5 ’untranslated region (5’UTR) polymerase chain reaction (PCR) product (-) after the HCV viral load was determined by the HCV RNA quantitation assay (Roche Diagnostic Systems) 274 to -31 nucleotides) and further amplified with a pair of nested PCR primers Geno-5 (-27 to -252 nucleotides) and Geno-6 (-64 to -43 nucleotides). Nested PCR products were sequenced using primers Geno-7 (-210 to -189 nucleotides) with only 5 nucleotide positions (-167, -163, -161, -160, -99 nucleotides) Six genotypes can be distinguished. Study selected 162 chronic C