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为查明广西田东县龙须河某养殖户网箱养殖黄颡鱼大批死亡的病原菌及其耐药性。以常规方法进行细菌分离,人工感染试验确定病原菌,API 20NE半自动细菌鉴定系统进行细菌鉴定,纸片扩散法进行药敏试验。试验结果,从具有典型发病症状的黄颡鱼中共分离到编号为TDA1、TDA2、TDB1、TDB2、TDB3的5株优势菌,对健康黄颡鱼的平均致死率分别为100%、100%、70.0%、65.0%和72.5%,表明均为引起黄颡鱼发病死亡的病原菌。经API 20NE鉴定,5株病原菌均为嗜水气单胞菌。根据药敏试验结果,25种试验药物中,对5株菌株都达到高度敏感的有环丙沙星、多粘菌素B、盐酸沙拉沙星、氧氟沙星、氟苯尼考、磺胺二甲嘧啶等6种,都耐药的有青霉素G和氨苄青霉素2种。
In order to find out the pathogen and drug-resistance of a large number of yellow catfish cultured in a farmer ’s cage of Longxue River in Tiandong County, Guangxi Province. Bacteria were isolated by routine methods. Pathogenic bacteria were identified by artificial infection test. API 20NE semi-automatic bacterial identification system was used for bacterial identification and disk diffusion method for susceptibility testing. The results showed that the average lethal rates of five dominant strains numbered TDA1, TDA2, TDB1, TDB2 and TDB3 were 100%, 100% and 70.0 respectively from yellow catfish with typical symptoms. %, 65.0% and 72.5% respectively, which indicated that all of them were pathogenic bacteria that caused the death of yellow catfish. The API 20NE identified five pathogens are Aeromonas hydrophila. According to the drug susceptibility test results, out of the 25 tested drugs, 5 strains were highly sensitive with ciprofloxacin, polymyxin B, sarafloxacin hydrochloride, ofloxacin, florfenicol, sulfonamide Pyrimidine and other 6 species are resistant to penicillin G and ampicillin 2 species.