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目的:探讨健脾清热化瘀中药对大鼠乙酸性胃溃疡愈合质量的影响。方法:采用冰醋酸注射法制备大鼠胃溃疡模型,随机分为模型组、雷尼替丁组(西药组)、健脾中药组、清热中药组、化瘀中药组、健脾清热化瘀中药组(合用组)和正常对照组(空白组)。于给药14 d后在光镜下观察溃疡指数(UI)、再生黏膜厚度、黏膜肌层缺损宽度及囊状扩张腺体数量,应用ELISA法检测血清表皮生长因子(EGF)及6-酮-前列腺素F1α(6-keto-PGF1α)含量,免疫组化法检测胃黏膜表皮生长因子受体(EGFR)的表达。结果:健脾、清热、化瘀中药及其合方都能使再生黏膜厚度增加,黏膜肌层缺损宽度及囊状扩张腺体数量减少,与模型组比较P<0.01,中药合方组疗效明显优于西药组(P<0.05或P<0.01)。健脾清热化瘀中药可明显提高血清6-keto-PGF1α含量,与模型组比较P<0.01,接近空白组水平,明显高于雷尼替丁组(P<0.05)。中药各组及西药组血清EGF含量较模型组及空白组升高(P<0.01),且可明显增强胃溃疡黏膜EGFR的表达(P<0.05或P<0.01),尤以中药合方组疗效最为明显(P<0.01)。结论:健脾清热化瘀中药通过改善胃溃疡再生黏膜组织学成熟度和功能成熟度,可明显改善实验性胃溃疡愈合质量,有效减少复发。
Objective: To investigate the effect of Chinese herbal medicine Jianpi Qingre Huayu on healing quality of gastric ulcer in rats. Methods: The rats model of gastric ulcer was prepared by injection of glacial acetic acid. The rats were randomly divided into model group, ranitidine group (western medicine group), Chinese medicine group of invigorating the spleen, traditional Chinese medicine group of clearing heat, traditional Chinese medicine group of removing blood stasis, Group (combined group) and normal control group (blank group). The ulcer index (UI), the thickness of regenerated mucosa, the width of muscularis mucosa defect and the number of cystic dilatation glands were observed under light microscope 14 days after the administration. Serum epidermal growth factor (EGF) and 6-keto- The content of prostaglandin F1α (6-keto-PGF1α) and the expression of epidermal growth factor receptor (EGFR) in gastric mucosa were detected by immunohistochemistry. Results: The effects of invigorating the spleen, clearing away heat and removing blood stasis and its combination on the thickness of regenerative mucosa increased, the width of mucosa defect and the number of cystic dilatation glands decreased, P <0.01 compared with the model group Better than Western medicine group (P <0.05 or P <0.01). The Chinese herbal medicine for invigorating the spleen, clearing away heat and removing blood stasis could obviously increase the content of 6-keto-PGF1α in serum, which was significantly lower than that in the model group (P <0.01), close to the level in the blank group and significantly higher than that in the ranitidine group (P <0.05). The content of EGF in each group and Western medicine group was higher than that in model group and blank group (P <0.01), and could significantly increase the expression of EGFR in gastric ulcer mucosa (P <0.05 or P <0.01), especially in traditional Chinese medicine group The most obvious (P <0.01). Conclusion: Jianpi Qingre Huayu Herbs can improve the quality of experimental gastric ulcer healing and reduce recurrence by improving histological maturation and maturation of gastric mucosa.