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目的:探讨丹参药材、脂溶性及水溶性提取物的UPLC-ESI-MS行为及相关性。方法:采用UPLC-ESI-MS技术,比较丹参药材、脂溶性及水溶性提取物的色谱及质谱行为,分析指标成分的裂解规律,检定其主要化合物。结果:指标成分丹参酮ⅡA及丹酚酸B在不同碰撞能量下,碎片的形成具有一定的规律性,丹参酮ⅡA的ESI-MS裂解特征是正离子模式下出现碎片m/z:280、277、262、252、249;丹酚酸B的ESI-MS裂解特征是负离子模式下形成m/z:519、321、339、295的碎片离子。丹参药材中检定出20个主要化合物,水溶性及脂溶性提取物中分别检定出11个和16个化合物。结论:采用UPLC-ESI-MS技术可用于同时测定丹参中水溶性及脂溶性成分,为进一步研究药材及提取物之间的化学成分相关性提供新的途径。
OBJECTIVE: To investigate the UPLC-ESI-MS behavior and its correlation of Radix Salviae Miltiorrhizae, fat-soluble and water-soluble extracts. Methods: The chromatographic and mass spectrometric behavior of Radix Salviae Miltiorrhizae Radix et Rhizoma Rhei were compared by UPLC-ESI-MS. The cleavage patterns of the constituents were analyzed and the main compounds were identified. Results: The tanshinone ⅡA and salvianolic acid B had some regularities of fragment formation under different collision energies. The ESI-MS of tanshinone ⅡA was characterized by fragment m / z 280,277,262 in positive ion mode, 252,249; ESI-MS cleavage of salvianolic acid B is a fragment ion that forms m / z: 519, 321, 339, 295 in negative ion mode. Radix Salviae Miltiorrhizae was identified in 20 major compounds, water-soluble and fat-soluble extracts were detected in 11 and 16 compounds. Conclusion: The UPLC-ESI-MS technique can be used to simultaneously determine water-soluble and fat-soluble components of Radix Salviae Miltiorrhizae, and provide a new way to further study the chemical components of Radix Salviae Miltiorrhizae.