The rapid and reliable measurement of hydrogen peroxide(H_2O_2) is imperative for many areas of technology, including pharmaceutical, clinical, food industry and environmental applications. In this work, a novel multifunctional complex, [Ru(bpy)_2(luminol-bpy)](PF_6)_2(bpy: 2,20-bipyridine), was designed and synthesized by incorporating a Ru(II) complex with a luminal group. In the presence of horseradish peroxidase(HRP), reaction of [Ru(bpy)_2(luminol-bpy)]_2+with H_2O_2 can be monitored by three sensing channels including photoluminescence(PL), chemiluminiscence(CL) and eletrochemiluminiscence(ECL). The quantitative assays for H_2O_2 in aqueous solutions using [Ru(bpy)_2(Luminalbpy)](PF_6)_2as a probe were established with PL, ECL and CL signal output modes, respectively. The rapid and reliable measurement of hydrogen peroxide (H_2O_2) is imperative for many areas of technology, including pharmaceutical, clinical, food industry and environmental applications. In this work, a novel multifunctional complex, [Ru (bpy) _2 (luminol-bpy) ] (PF_6) _2 (bpy: 2,20-bipyridine) was designed and synthesized by incorporating a Ru (II) complex with a luminal group. In the presence of horseradish peroxidase (HRP), reaction of [Ru (luminol-bpy)] _2 + with H_2O_2 can be monitored by three sensing channels including photoluminescence (PL), chemiluminiscence (CL) and eletrochemiluminiscence (ECL). The quantitative assays for H_2O_2 in aqueous solutions using [Ru (bpy) _2 (Luminalbpy )] (PF_6) _2as a probe were established with PL, ECL and CL signal output modes, respectively.