论文部分内容阅读
目的:观察人参皂苷Rg_1预处理对异丙肾上腺素致急性心肌缺血大鼠心肌磷酸肌醇3-激酶(PI3K)/蛋白激酶B(PKB/Akt)通路的影响。方法:采用异丙肾上腺素建立SD大鼠急性心肌缺血模型,将50只大鼠随机分为正常组,模型组,葛根素组,人参皂苷Rg_1高、低剂量组(20,10 mg·kg~(-1));Moor激光血流成像系统观测各组大鼠心脏表面血流值;酶联免疫吸附测定(ELISA)法测定各组大鼠血清中肌酸激酶(CK),乳酸脱氢酶(LDH),一氧化氮(NO)的水平以及心肌中超氧化物歧化酶(SOD),丙二醛(MDA),谷胱甘肽过氧化物酶(GSH-Px)的水平;实时荧光定量-聚合酶链式反应(Real-time PCR)法检测各组大鼠心肌内皮型一氧化氮合酶(eNOS)mRNA的表达变化;蛋白质免疫印迹(Western blot)方法检测各组大鼠心肌PI3K,Akt,p-Akt蛋白的表达变化。结果:与正常组比较,模型组大鼠心脏表面平均血流量明显下降,血清NO降低,CK,LDH升高;心肌MDA含量升高,GSH-Px含量下降,eNOS mRNA含量显著降低,PI3K/Akt通路蛋白的表达有所降低(P<0.05,P<0.01);与模型组比较,人参皂苷Rg_1高、低剂量组,心肌表面平均血流有显著提升,血清NO升高,CK,LDH降低,心肌MDA含量降低,GSH-Px含量升高,eNOS mRNA表达含量升高,PI3K/Akt通路蛋白的表达有所升高(P<0.05,P<0.01)。结论:人参皂苷Rg_1可以通过调控PI3K-Akt-eNOS信号通路改善急性心肌缺血大鼠心脏变化防治心血管病变。
Objective: To observe the effect of ginsenoside Rg 1 preconditioning on myocardial phosphoinositide 3-kinase (PI3K) / protein kinase B (PKB / Akt) pathway induced by isoprenaline in acute myocardial ischemia rats. Methods: Acute myocardial ischemia model of SD rats was established by isoprenaline. Fifty rats were randomly divided into normal group, model group, puerarin group, high and low dose ginsenoside Rg_1 group (20 and 10 mg · kg -1) ~ (-1)). The blood flow in the heart of each group was observed by Moor laser blood flow imaging system. The levels of creatine kinase (CK), lactate dehydrogenation (LDH), nitric oxide (NO), as well as the level of myocardial SOD, MDA and GSH-Px, and real-time fluorescence quantitative The expression of eNOS mRNA in myocardium of each group was detected by Real-time PCR. Western blot was used to detect the expression of PI3K, Akt, p-Akt protein expression changes. Results: Compared with the normal group, the mean blood flow in the heart of the model group decreased significantly, the level of serum NO decreased and the level of CK and LDH increased. The content of MDA increased, the content of GSH-Px decreased, the content of eNOS mRNA decreased, the level of PI3K / (P <0.05, P <0.01) .Compared with the model group, the average blood flow of myocardium in high and low dose ginsenoside Rg_1 groups increased significantly, serum NO increased, CK and LDH decreased, Myocardial MDA content decreased, GSH-Px content increased, eNOS mRNA expression increased, PI3K / Akt pathway protein expression increased (P <0.05, P <0.01). CONCLUSION: Ginsenoside Rg_1 can prevent and treat cardiovascular diseases by ameliorating PI3K-Akt-eNOS signal pathway in ameliorating cardiac changes in acute myocardial ischemia rats.