论文部分内容阅读
目的 :研究胰岛素 (insulin ,Ins)对家兔急性缺血再灌注性肾损伤的影响。方法 :采用钳夹肾动脉的方法建造急性肾缺血再灌注肾损伤模型。实验动物分为三组 :对照组、单纯缺血再灌注 (IR)组、胰岛素处理 (Ins -IR)组。胰岛素处理组再灌注的同时给予胰岛素溶液 (含Ins 3UI/kg ,葡萄糖 1.5 g/kg ,k+ 0 .4mg/kg) ,对照组和IR组给予等量生理盐水。分别观察三组动物缺血再灌注 2h ,4 8h后 ,血清尿素氮 (BUN)、血糖、血清及肾组织中丙二醛 (MDA)、肾组织一氧化氮 (NO)含量变化 ,以及肾组织超微结构改变。结果 :肾缺血再灌注 4 8h后 ,IR组血清尿素氮较对照组显著升高 (P <0 .0 0 1) ,而Ins-IR组与对照组无显著差异 (P >0 .0 5 ) ;IR组血清及肾组织中MDA含量较对照组显著升高 (P <0 .0 5 ) ,胰岛素处理后MDA含量明显降低 (P <0 .0 5 ) ;缺血再灌注 2h后 ,肾组织中NO含量即明显降低 (P <0 .0 0 1) ,胰岛素处理后 ,NO水平升高至正常水平。缺血再灌注 2h后 ,三组动物血糖均较术前增高 ,但以IR组增高更为显著 ,与对照组比较P <0 .0 5 ,Ins-IR组与对照组无差异。电镜结果显示 ,对照组超微结构正常 ,IR组肾组织呈变性和坏死改变 ,Ins -IR组肾组织轻度变性。结论 :胰岛素具有抗家兔急性缺血再灌注性肾损伤的作用 ,其作用?
Objective: To study the effect of insulin on acute renal ischemia-reperfusion injury in rabbits. Methods: Acute renal ischemia / reperfusion injury model was established by clipping the renal artery. The experimental animals were divided into three groups: control group, IR group and Ins-IR group. Insulin treatment group was given insulin solution (including Ins 3UI / kg, glucose 1.5g / kg, k + 0.4mg / kg) while reperfusion. The control group and IR group were given the same amount of saline. The changes of serum urea nitrogen (BUN), blood glucose, malondialdehyde (MDA) in serum and kidney tissue and the content of nitric oxide (NO) in kidney were observed after ischemia and reperfusion for 2h and 48h respectively in three groups, Ultrastructural changes. RESULTS: After 48 h of renal ischemia-reperfusion, serum urea nitrogen in IR group was significantly higher than that in control group (P <0.01), but there was no significant difference between Ins-IR group and control group (P> 0.05) ); The content of MDA in serum and renal tissue of IR group was significantly higher than that of control group (P <0.05), the content of MDA in insulin treated group was significantly decreased (P <0.05); after 2h of ischemia reperfusion, The content of NO in the tissue was significantly decreased (P <0.01). After insulin treatment, the NO level increased to the normal level. After 2h of ischemia-reperfusion, the blood glucose of the three groups of animals increased compared with the preoperative level, but increased more significantly in the IR group compared with the control group (P <0.05). There was no difference between the Ins-IR group and the control group. Electron microscopy results showed that the ultrastructure of the control group was normal, the renal tissue of the IR group was changed into degeneration and necrosis, and the renal tissue of the Ins-IR group was slightly degenerated. Conclusion: Insulin has anti-ischemic reperfusion kidney injury in rabbits, its role?