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In order to understand in vitro antibacterial effect of antibiotics and EM on Aeromonas hydrophila,14 isolates of A. hydrophila were isolated from diseased fish and aquaculture in the study. PCR amplification results of aerolysin gene( Aero gene) of isolates indicated that 7 out of 14 isolates were pathogenic A.hydrophila. In vitro antibacterial test indicated that 14 isolates were resistance to penicillin G,ampicillin,cephradine,cefazolin,tetracycline,terimethoprim,lincomycin and cephalexin,but showed high sensitivity to cefotaxime and furazolidone. In vitro antibacterial effect of EM on 14 isolates of A. hydrophila was positively correlated with concentration,and EM with the concentration greater than 60% had antibacterial effect on 14 isolates.
In order to understand in vitro antibacterial effect of antibiotics and EM on Aeromonas hydrophila, 14 isolates of A. hydrophila were isolated from diseased fish and aquaculture in the study. PCR amplification results of aerolysin gene (Aero gene) of isolates indicated that 7 out of 14 isolates were pathogenic A. hydrophila. In vitro antibacterial test indicated that 14 isolates were resistance to penicillin G, ampicillin, cephradine, cefazolin, tetracycline, terimethoprim, lincomycin and cephalexin, but showed high sensitivity to cefotaxime and furazolidone. In vitro antibacterial effect of EM on 14 isolates of A. hydrophila was positively correlated with concentration, and EM with the concentration greater than 60% had antibacterial effect on 14 isolates.