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目的探讨矾冰纳米乳治疗烧伤的可能作用机制。方法将表皮干细胞分为中药低、中、高剂量组和正常组、对照组,中药低、中、高剂量组分别加入8.15、16.3、32.6 mg/L的矾冰纳米乳1 ml,对照组加入湿润烧伤膏1 ml,正常组单纯加入Defined K-SFM培养基1 ml。用RT-PCR、免疫印迹法分别在第24 h及3、5、7天检测表皮干细胞骨桥蛋白及其mRNA表达。结果 RT-PCR检测结果显示,与对照组比较,中药中、高剂量组各时间点骨桥蛋白mRNA表达均明显降低(P<0.05),且明显低于同时间的低剂量组(P<0.05)。Western blot检测结果显示,与对照组比较,第3、5、7天中药中、高剂量组骨桥蛋白表达明显降低(P<0.05);与中药低剂量组比较,第3、5、7天中药中、高剂量组骨桥蛋白表达明显降低(P<0.05)。结论矾冰纳米乳治疗烧伤可能与调控表皮干细胞骨桥蛋白基因的表达有关,并且以8.15 mg/L浓度最优。
Objective To investigate the possible mechanism of alum ice nanoemulsion in the treatment of burn. Methods Epidermal stem cells were divided into low, medium and high dose of traditional Chinese medicine group and normal group, control group, traditional Chinese medicine low, medium and high dose group were added 8.15,16.3,32.6 mg / L alum ice nanoemulsion 1 ml, control group Wet burn cream 1 ml, normal group simply add Defined K-SFM medium 1 ml. The expressions of osteopontin and its mRNA in epidermal stem cells were detected by RT-PCR and Western blot at 24 h and 3, 5 and 7 days respectively. Results The results of RT-PCR showed that the expression of osteopontin mRNA in middle-dose and high-dose groups were significantly lower than those in the control group (P <0.05), and significantly lower than those in the low-dose group ). Western blot results showed that compared with the control group, the osteopontin expression was significantly decreased in the medium and high dose groups on the 3rd, 5th and 7th day (P <0.05). Compared with the low dose group, The expression of osteopontin in middle and high dose of traditional Chinese medicine group was significantly decreased (P <0.05). Conclusion Alum ice nanoemulsion treatment of burn injury may be related to the regulation of osteopontin gene expression in epidermal stem cells, and the optimal concentration of 8.15 mg / L.