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目的 研究晶体蛋白能否促进离体培养的视网膜神经节细胞 (RGCs)存活和生长,初步探讨损伤晶状体促进RGCs的存活和轴突再生的确切作用物质。 方法 分别用晶体蛋白、晶体蛋白激活的巨噬细胞培养液及DMEM对RGCs进行培养,观察RGCs在体外存活时间,测量培养 1, 3, 5d有突起RGCs数、最长突起长度及细胞活性,进行组间比较。 结果 (1)晶体蛋白组细胞能存活 12~14d,较其他两组显著延长 (P<0. 05 )。 ( 2 )培养 1, 3, 5d,各实验组有突起的RGCs数均明显多于对照组(P<0. 01),晶体蛋白组有突起的RGCs数较其激活的巨噬细胞培养液组明显增多(P<0. 01)。(3)培养 1, 3, 5d,各实验组RGCs最长突起长度均较对照组显著延长 (P<0. 01),且晶体蛋白组与其激活的巨噬细胞培养液组比较,差异有统计学意义 (P<0. 01)。(4)培养 3d和 5d时,晶体蛋白组细胞活性显著高于对照组 (P<0. 01)。 结论 (1)晶体蛋白对离体培养的RGCs存活和生长具有显著的直接促进作用; (2)晶体蛋白也可通过激活巨噬细胞间接发挥作用,但以直接作用为主。
Objective To investigate whether crystal protein can promote the survival and growth of cultured retinal ganglion cells (RGCs) in vitro and to explore the exact role of lens in promoting RGCs survival and axonal regeneration. Methods RGCs were cultured with crystal protein, crystal protein-activated macrophages and DMEM respectively. The survival time of RGCs was observed in vitro. The number of RGCs, the length of the longest neurites and the cell viability were measured at 1, 3 and 5 days after culture Comparison between groups. Results (1) The crystalloid cells survived for 12-14 days, significantly longer than the other two groups (P <0.05). (2) After cultured for 1, 3, and 5 days, the number of RGCs in each experimental group was significantly higher than that in the control group (P <0.01). The numbers of RGCs in the crystal protein group were significantly higher than those in the macrophage culture group Significantly increased (P <0.01). (3) After cultured for 1, 3 and 5 days, the longest protuberance length of RGCs in each experimental group was significantly longer than that of the control group (P <0.01), and there was statistical difference between the crystal protein group and the activated macrophage culture medium group Significance of learning (P <0.01). (4) The cell activity of crystal protein group was significantly higher than that of the control group at 3d and 5d (P <0.01). Conclusion (1) Crystal protein has a direct and direct effect on the survival and growth of RGCs cultured in vitro. (2) Crystal protein can also play an indirect role by activating macrophages, but mainly by direct effect.