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目的:建立HPLC-DAD法同时分析测定五加皮保健酒综合提取液中6个成分(阿魏酸、肉桂酸、桂皮醛、甘草酸、木香烃内酯、去氢木香内酯)的方法。方法:采用Eclipse XD8-C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.05%磷酸水溶液(B)为流动相,梯度洗脱(0~8 min,12%A;8~10 min,12%→19%A;10~37 min,19%→50%A;37~57 min,50%→70%A;57~60 min,70%→12%A),流速为1.0 mL/min,柱温为30℃,检测波长为225 nm(去氢木香内酯、木香烃内酯)、237 nm(甘草酸)、278 nm(肉桂酸、桂皮醛)、316 nm(阿魏酸)。结果:阿魏酸、肉桂酸、桂皮醛、甘草酸、木香烃内酯、去氢木香内酯均能达到良好分离,在线性范围内线性关系良好(r2≥0.0097),精密度RSD≤0.71%,24h内稳定性试验RSD≤1.8%,各成分平均加样回收率在98.2%~102.8%,RSD值在1.0%~1.7%。结论:该方法操作简单,重复性好,为五加皮保健酒的质量控制提供可靠的方法。
OBJECTIVE: To establish a simultaneous HPLC-DAD method for the simultaneous determination of six components (ferulic acid, cinnamic acid, cinnamic aldehyde, glycyrrhizic acid, wood ketone lactone, dehydrocostus lactone) in the comprehensive extract of Wujiapi health wine method. Methods: The mobile phase was eluted with gradient elution (0 ~ 8 min, 12% A, 8%) using Eclipse XD8-C18 (250 mm × 4.6 mm, 5 μm) ~ 10 min, 12% → 19% A; 10 ~ 37 min, 19% → 50% A; 37 ~ 57 min, 50% → 70% A; 57 ~ 60 min, 70% → 12% 1.0 mL / min, the column temperature was 30 ℃, the detection wavelength was 225 nm (dehydrocostuslactone), 237 nm (glycyrrhizic acid), 278 nm (cinnamic acid) (Ferulic acid). RESULTS: Ferulic acid, cinnamic acid, cinnamaldehyde, glycyrrhizic acid, wood ketone lactone and dehydrocostuslactone all achieved good separation with good linearity (r2≥0.0097) in the linear range. The precision RSD≤ 0.71%, 24h RSD stability test ≤ 1.8%, the average recovery of each component was 98.2% ~ 102.8%, RSD value of 1.0% to 1.7%. Conclusion: The method is simple, reproducible and provides a reliable method for the quality control of Wujiapi health wine.