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目的建立9L/F344大鼠颅内脑胶质瘤模型,用增强MRI动态观察肿瘤生长。方法应用大鼠立体定向仪,在F344大鼠右侧尾状核区接种9L胶质瘤细胞1×105个;观察大鼠的生存状态、体重及活动等情况;分别于大鼠接种后第8、12、17、20、23天在小动物线圈下行大鼠头部强化MRI检查,动态观察肿瘤的生长情况;第20天,取肿瘤标本做组织病理学和神经胶质细胞纤维酸性蛋白(GFAP)免疫组化检查。结果大鼠平均生存期25d。大鼠成瘤率100%。增强MRI动态观察显示同一时期内大鼠的肿瘤体积均一,肿瘤的生长速度和形态符合胶质瘤生长特性;肿瘤组织病理学上接近人恶性脑胶质瘤的病理特点。结论该方法建立的9L/F344大鼠脑胶质瘤动物模型稳定性好,移植瘤生长均一,适合用于胶质瘤的基础研究。
Objective To establish an intracranial glioma model of 9L / F344 rats and observe the tumor growth with enhanced MRI. Methods Rat stereotactic apparatus was used to inoculate 1 × 105 9L glioma cells in the right caudate nucleus of F344 rats. The survival status, body weight and activity of rats were observed. On the 12th, 20th, 20th, 20th, 20th and 23th days, the head of rats were examined by MRI in the lower animals’ coils, and the growth of tumor was observed dynamically. On the 20th day, the tumor samples were taken for histopathology and glial fibrillary acidic protein Immunohistochemistry. Results The average survival time of rats 25d. Rat tumor formation rate of 100%. The dynamic MRI showed that the tumor volume of rats in the same period was uniform and the growth rate and morphology of the tumor were in line with the growth characteristics of glioma. The histopathology of tumor was close to the pathological features of human malignant glioma. Conclusion The animal model of 9L / F344 rat glioma established by this method has good stability and uniform growth of xenografts, which is suitable for the basic research of glioma.