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目的研究4株阴道毛滴虫对小鼠组织的侵袭力。方法取有症状的阴道毛滴虫患者的阴道后穹窿分泌物,无菌培养,获得的4株阴道毛滴虫各感染30只昆明小鼠,每只经腹腔接种0.5ml(含5×106个虫体/ml)的阴道毛滴虫悬液,每天观察小鼠的行为变化。分别于第10、20、30d剖杀小鼠,剥开腹腔,检查各器官病理改变。结果共接种小鼠120只,解剖107只,检出有病变小鼠37只,占30.83%。小鼠接种滴虫后,外观行为变化包括行动迟缓、精神萎靡、竖毛、腹胀等。发生病变的小鼠腹腔脏器表面可见多处脓肿,病变脏器中均有活滴虫,受累脏器涉及肝、脾、肾、胰、胃、睾丸、卵巢等。观察病变肝组织病理变化,可见滴虫在肝脏形成脓肿,引起变性、坏死。病变早期,肝细胞间隙或溶解的组织中可见椭圆形游离的滴虫,也有滴虫附着于组织细胞上,紧密排成一列,呈栅栏状;病变中期,在坏死的病变区可见数量多、密集成团的滴虫;晚期,变性肝细胞围绕脓肿腔呈索状排列,肝细胞核皱缩,发生玻璃样变或溶解。结论阴道毛滴虫对小鼠组织有较强的侵袭力和致病性,滴虫接触并粘附组织细胞在其致病过程中起重要作用。感染小鼠的器官发生一系列的病理变化。
Objective To study the invasiveness of 4 strains of Trichomonas vaginalis on mouse tissues. Methods The vaginal vault secretions were collected from patients with symptomatic vaginal Trichomonas vaginalis, and each of the four strains of Trichomonas vaginalis were infected with Kunming mice by intraperitoneal inoculation with 0.5 ml (containing 5 × 106 Parasites / ml) of Trichomonas vaginalis suspension, mice were observed daily behavioral changes. Mice were sacrificed on the 10th, 20th and 30th days, respectively. The abdominal cavity was dissected and pathological changes of various organs were examined. Results A total of 120 mice were inoculated and 107 were dissected. There were 37 diseased mice, accounting for 30.83%. Mice vaccinated with trichomoniasis, the appearance of behavioral changes include slow, apathetic, vertical hair, abdominal distension and so on. Lesions in mice abdominal cavity surface can be seen multiple abscesses, organ disease lesions are living Trichomonas, Involved organs involved in liver, spleen, kidney, pancreas, stomach, testes, ovaries and so on. Observe the pathological changes of liver lesions, showing that trichomoniasis in the liver abscess, causing degeneration and necrosis. Early lesions, hepatocytes interstitial or dissolved tissue can be seen oval-free trichomonas, trichomoniasis also attached to the tissue cells, closely arranged in a column, was fence-like; mid-lesion, visible in the lesion area of the number of more dense Into the group of trichomoniasis; late, degeneration of liver cells around the cord-shaped arrangement of abscess, liver cell shrinkage, the occurrence of glassy change or dissolution. Conclusion Trichomonas vaginalis has strong invasiveness and pathogenicity on mouse tissues. Trichomonas vaginalis contacts and adheres to the tissue cells in the pathogenic process plays an important role. Infected mice organs undergo a series of pathological changes.