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为研究光合促进剂NaHSO3促进油菜叶片光合的作用机理,以甘蓝型油菜中双11号为材料,在水培条件下研究了不同浓度NaHSO3(0~0.08mmol·L-1)处理对五叶期幼苗生长发育、氮代谢及光合荧光特性的影响。结果表明,水培条件下,低浓度(0.02mmol·L-1)NaHSO3处理10d、15d和20d后植株株高均显著增加,分别比对照提高21.0%、28.4%和40.4%;鲜重和根长虽有增加但与对照相比差异不显著;高浓度(0.08mmol·L-1)NaHSO3处理10~20d后显著抑制植株根系长度,抑制率为15.1%~16.5%。不同NaHSO3浓度处理均显著增加了叶绿素b和总叶绿素含量,其中0.02mmol·L-1NaHSO3处理对叶绿素含量的促进作用最大,且只有该浓度处理会显著提高叶绿素a含量;NaHSO3处理油菜后,净光合速率、光饱和点和表观量子效率显著增加,光呼吸速率显著降低;最大光能转化率(Fv/Fm)和实际光能转化率(ΦPSⅡ)增加,非光化学猝灭系数(NPQ)降低;不同NaHSO3浓度处理均能增加硝酸还原酶(NR)和谷氨酰胺合成酶(GS)的活性,其中对NR的促进作用在0.02mmol·L-1达到最高。本研究结果表明,NaHSO3对光合速率的促进作用与光呼吸抑制无关,而是主要通过促进油菜叶片中叶绿素b含量增加,进而提高了光能吸收和传递效率,因此光合速率提高。
In order to study the mechanism of photosynthesis promoting photosynthesis of rape leaves, NaHSO3 was used to study the effects of different concentrations of NaHSO3 (0 ~ 0.08 mmol·L-1) Effects of Seedling Growth and Development, Nitrogen Metabolism and Photosynthesis Fluorescence Properties. The results showed that under hydroponic culture, plant height at 10d, 15d and 20d after treated with 0.02mmol·L-1 NaHSO3 was significantly increased by 21.0%, 28.4% and 40.4%, respectively. Fresh weight and root The length of roots was increased but the difference was not significant compared with the control. The roots of plants were significantly inhibited after treated with 0.08 mmol·L-1 NaHSO3 for 10-20 days with the inhibition rate of 15.1% -16.5%. The concentrations of chlorophyll b and total chlorophyll increased significantly under different concentrations of NaHSO3, of which 0.02 mmol·L-1NaHSO3 promoted chlorophyll content, and only chlorophyll-a content was significantly increased after treatment with NaHSO3. The maximum light energy conversion (Fv / Fm) and the actual light energy conversion rate (ΦPSⅡ) increased while the non-photochemical quenching coefficient (NPQ) decreased. The activities of nitrate reductase (NR) and glutamine synthetase (GS) were increased with different concentrations of NaHSO3, and the promotion of NR reached the highest at 0.02mmol·L-1. The results showed that NaHSO3 had no effect on photosynthetic rate, but increased photosynthetic rate by increasing the content of chlorophyll b in rapeseed leaves, thus increasing the light energy absorption and transfer efficiency.