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目的探讨2009年山东省甲型H1N1流感病毒神经氨酸酶(neuram inidase,NA)基因的进化及NA基因编码蛋白抗原性、酶活性位点、糖基化位点变异情况。方法对山东省分离的20株甲型H1N1流感病毒的NA基因序列,用MEGA 4.0软件进行基因进化分析和氨基酸序列分析。结果山东省2009年9月-2010年1月分离的20株甲型H1N1流感病毒NA基因的同源性均>99.2%,其中有4株的同源性为100%;与疫苗株[A-Californ ia-07-2009(H1N1)]、国内推荐株[A-S ichuan-SWL1-2009(H1N1)]的同源性分别为99.1%~99.5%和99.1%~99.6%;有13个神经氨酸酶基因的氨基酸发生了替换,为21、23、25、42、43、60、76、94、106、122、248、307和351位;未发生神经氨酸酶蛋白275位H-Y的替换。结论山东省分离的甲型H1N1流感病毒NA基因高度保守,潜在抗原位点氨基酸分布相同;所有毒株的酶活性中心位点以及糖基化位点均高度保守;所有测定样本均未发生对达菲类药物的耐药性突变。
Objective To investigate the evolution of neuram inidase (NA) gene of influenza A (H1N1) virus in Shandong Province in 2009 and the variation of antigenicity, enzyme activity and glycosylation sites of NA protein. Methods The NA gene sequence of 20 strains of influenza A (H1N1) viruses isolated in Shandong Province was analyzed by MEGA 4.0 software and analyzed by amino acid sequence. Results The homologies of NA gene of 20 strains of influenza A (H1N1) isolates isolated from September 2009 to January 2010 in Shandong Province were all> 99.2%, of which 4 strains had homology of 100% Californ ia-07-2009 (H1N1)] and the homologs of the domestic recommended strain [ASichuan-SWL1-2009 (H1N1)] were 99.1% -99.5% and 99.1% -99.6%, respectively. Thirteen neuraminidase The amino acids of the gene were replaced at 21, 23, 25, 42, 43, 60, 76, 94, 106, 122, 248, 307 and 351; no substitution of the 275 amino acids of the neuraminidase protein occurred. Conclusion The NA gene of Influenza A (H1N1) virus isolated in Shandong Province is highly conserved and the amino acid distributions of potential antigenic sites are the same. All the enzyme activity centers and glycosylation sites are highly conserved; Phenotypic drug resistance mutations.