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[目的]在光合膜水平上对两优培九倒二、倒三叶的光能转化特性进行研究,进一步探讨功能叶倒二、倒三在抽穗期的生理变化,以揭示不同衰老程度叶片的光合功能。[方法]以抽穗期的两优培九为材料,测定倒二、倒三叶的叶绿素、类胡萝卜素、可溶性蛋白和可溶性总糖含量以及类囊体膜PSⅠ、PSⅡ电子传递活性、类囊体膜室温吸收光谱、类囊体膜室温发射光谱等指标。[结果]倒二、倒三叶的叶绿素和类胡萝卜素含量随采样时间的延长而下降;可溶性蛋白含量、可溶性总糖含量都下降,但倒三叶下降幅度比倒二叶大;PSⅠ电子传递活性都在不断下降,PSⅡ电子传递活性则先上升后下降;类囊体膜室温吸收光谱都是先上升后下降,两者的室温发射光谱则是先下降后上升。[结论]功能叶倒三在抽穗期的生理变化比倒二更明显,叶片的光合功能比倒二叶弱,衰老比倒二叶快。
[Objective] The research aimed to study the light energy conversion characteristics of Liangyou Peijiu inverted and inverted clover at the photosynthetic membrane level, and further discussed the physiological changes of functional leaves upside down and upside down at heading stage to reveal the photosynthetic function of leaves with different senescence. [Method] The contents of chlorophyll, carotenoid, soluble protein, total soluble sugar and the PS Ⅰ, PS Ⅱ electron transfer activity of thylakoid membrane and the absorption of thylakoid membranes at room temperature were measured by using heading date Liangyoupeijiu as material. Spectroscopy, thylakoid membrane emission spectroscopy and other indicators. [Result] The content of chlorophyll and carotenoid in the second and third leaves decreased with the increase of sampling time. The contents of soluble protein and total soluble sugar decreased, but the decline of inverted leaves was larger than that of the second leaves. The PS Ⅰ electron transfer The activity of PSⅡdecreased continuously and the activity of PSⅡelectron transport firstly increased and then decreased. The absorption spectra of thylakoid membrane at room temperature firstly increased and then decreased, while the emission spectra at room temperature of the thylakoid membrane firstly decreased and then increased. [Conclusion] Physiological changes of function leaves in heading stage were more obvious than those in leaves 2, photosynthetic function of leaves was weaker than that in leaves 2, and senescence was faster than leaves 2.