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通过观察移植物的细胞形态和检测移植后巴金森氏模型鼠旋转行为的变化,探讨了低温储存肾上腺髓质组织的可行性。肾上腺髓质组织取自成年Sprague--Dawley大鼠,以每分钟降温1～2℃的速率冰冻,在液氮中储存1周,然后移植于6羟多巴胺损毁一侧黑质的模型鼠尾状核内。移植后Apomorphine诱发模型鼠的旋转行为有不同程度改善;冰冻储存的肾上腺髓质组织在细胞形态和染色特性上未发生变化;荧光观察表明移植物仍然保持产生儿茶酚胺的机能。这提示低温冰冻没有改变肾上腺髓质组织的生物学特性,可应用冰冻储存肾上腺髓质组织的方法建立组织库。
The feasibility of cryopreserved adrenal medulla tissue was discussed by observing the morphological changes of the grafts and detecting the rotation behavior of the Parkinson’s mouse after transplantation. Adrenal medulla tissue was obtained from adult Sprague - Dawley rats, frozen at 1 ~ 2 ℃ per minute, stored in liquid nitrogen for 1 week and then transplanted in the caudate of the substantia nigra on the side of 6 - hydroxydopamine damage Nuclear. After the transplantation, the rotation behavior of Apomorphine-induced mice improved to some extent; the frozen stored adrenal medulla tissue did not change in cell morphology and staining characteristics; fluorescence observation showed that the graft still maintained the function of producing catecholamines. This suggests that cryogenic freezing does not change the biological characteristics of adrenal medulla tissue, frozen storage of adrenal medulla tissue can be used to establish tissue bank.