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[目的]研究白术和白头翁2种中药提取物的杀菌活性,为其在植物源农药上的开发利用奠定基础。[方法]采用菌丝生长速率法,研究了白术和白头翁2种植物提取物对番茄灰霉病菌和番茄早疫病菌的抗菌活性。[结果]供试2种中药提取物对番茄灰霉病菌和番茄早疫病菌均具有较好的抗菌活性,其中白头翁提取物的抗菌活性最好,72h白头翁正丁醇提取物对番茄灰霉病菌的抑制率达80.25%;白术石油醚提取物对番茄灰霉病菌的抗菌活性较好,EC50为5.31mg/ml,白头翁正丁醇提取物的抗菌活性较强,EC50为2.93mg/ml。[结论]白头翁提取物对番茄灰霉病菌和番茄早疫病菌的抑制活性较好,值得进一步研究。
[Objective] The research aimed to study the bactericidal activity of two kinds of traditional Chinese medicine extracts of Atractylodes macranthoides and Pulsatilla, laying the foundation for its exploitation and utilization on plant-derived pesticides. [Method] The mycelial growth rate method was used to study the antibacterial activity of two plant extracts of Atractylodes macranthoides and Pulsatilla on tomato gray mold fungi and wheat Alternaria solani. [Result] The two kinds of traditional Chinese medicine extracts had good antibacterial activities against Botrytis cinerea and Alternaria solani. The antibacterial activity of Pulsatillae extracts was the best, The inhibitory rate of Atractylodes macrocephala was 80.25%. The antibacterial activity of petroleum ether extract of Atractylodes macrocephala against Botrytis cinerea was better with an EC50 of 5.31mg / ml. Butanol extract of Pulsatilla chinensis had stronger antibacterial activity with EC50 of 2.93mg / ml. [Conclusion] Pulsatilla striatum extract had good inhibitory activity against Botrytis cinerea and Alternaria solani, which deserved further study.