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实验确定了新鲜灵芝孢子进行低能N+离子注入的适宜参数:注入剂量1.25×1016ions.cm-2,注入能量20 KeV,靶室真空度10-3Pa,以5 s脉冲式注入,间隔为15 s。离子注入后,筛选酯酶同工酶酶谱存在显著差异和拮抗实验呈阳性的突变菌株,分别以液体发酵菌丝体多糖含量、固体栽培子实体生物转化率为初筛和复筛标准,经遗传稳定性实验验证,筛选到了一株灵芝高产菌株GL1026,其灵芝子实体产量、多糖含量、三萜含量分别较对照菌株提高了15.94%、9.68%、17.60%。结果表明:低能N+离子束注入是一种较为理想的灵芝育种手段。
The optimum parameters for low energy N + ion implantation of fresh Ganoderma lucidum spores were determined experimentally: injection dose of 1.25 × 1016ions.cm-2, implantation energy of 20 KeV, target chamber vacuum of 10-3Pa, pulse injection of 5 s, with an interval of 15 s. After ion implantation, the mutant strains with significant difference in esterase isoenzyme profile and antagonistic test were screened, and the content of polysaccharide in liquid fermentation mycelium and the bioconversion rate of solid culture fruiting body were used as the primary screening and re-screening standards respectively. Genetic stability of the experiment confirmed that a strain of high-yielding Ganoderma lucidum strain GL1026 was screened. The yield, polysaccharide content and triterpene content of Ganoderma lucidum fruiting bodies were increased by 15.94%, 9.68% and 17.60% respectively compared with the control strains. The results show that low energy N + ion beam implantation is an ideal means of breeding Ganoderma lucidum.