人牙周膜成纤维细胞是牙周细胞生物学、药理学、毒理学和牙周组织工程学研究的基础,对口腔各个领域意义重大。由于受到牙周组织的解剖结构和取材数量的限制,牙周膜成纤维细胞的体外培养较为困难,因此本文就人牙周膜成纤维细胞的体外培养方法和应用等研究进展作一综述。 Human periodontal ligament fibroblasts are the basis of periodontal cell biology, pharmacology, toxicology, and periodontal tissue engineering and are of great interest in all aspects of the oral cavity. Due to the limitation of the periodontal tissue anatomical structure and the quantity of the extracted materials, the periodontal ligament fibroblasts are difficult to culture in vitro. Therefore, the research progress of the methods and applications of human periodontal ligament fibroblasts in vitro is reviewed in this paper.