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目的:利用基因芯片技术研究RNA干扰下调PPP2R5C对K562细胞中TP53通路相关基因表达的影响。方法:Affymetrix U133 plus 2.0基因表达谱芯片检测核转染PPP2R5C-siRNA991和SC对照组的K562细胞,应用Genespring GX 11.0软件分析差异基因相关信号通路变化情况。结果:基因芯片分析TP53信号通路紧密相关的22个基因,发现全部基因发生了差异表达,其中上调基因有8个,下调基因有14个。明显上调的基因有EP300、CCND1、BRCA2和USP7,而明显下调的基因则有MDM4、ESR1、ATM、CDK2、CDKN1A、TP53和CDKN2A。结论:下调K562细胞PPP2R5C基因时,在一定程度上选择性调节与细胞增殖密切相关的TP53信号通路相关基因的表达,从而抑制K562细胞增殖,促进了K562细胞凋亡。
OBJECTIVE: To study the effect of down-regulation of PPP2R5C on the expression of TP53 pathway-related gene in K562 cells by RNAi. METHODS: K562 cells transfected with PPP2R5C-siRNA991 and SC control cells were detected by Affymetrix U133 plus 2.0 gene expression microarray. Genespring GX 11.0 software was used to analyze the changes of the differential gene-related signal pathways. RESULTS: Twenty-two genes closely related to the TP53 signaling pathway were analyzed by gene chips. All of the genes were differentially expressed, including 8 up-regulated genes and 14 down-regulated genes. Significantly up-regulated genes were EP300, CCND1, BRCA2 and USP7, while genes that were significantly down-regulated were MDM4, ESR1, ATM, CDK2, CDKN1A, TP53 and CDKN2A. CONCLUSION: Down-regulation of PPP2R5C gene in K562 cells selectively regulates the expression of TP53 signaling pathway-related genes that closely related to cell proliferation to a certain extent, thereby inhibiting the proliferation of K562 cells and promoting the apoptosis of K562 cells.