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目的调查2012年春季江苏省某县羊及其体表蜱中发热伴血小板减少综合征病毒(SFTSV)感染情况,并对分离到的毒株进行全基因序列分析。方法采用荧光定量RT-PCR法对标本进行SFTSV核酸检测,所有阳性标本进行病毒分离并测序,序列结果用Lasergene和MEGA4软件进行拼接和分析。结果 7只羊和从羊体上采集的93只长角血蜱中,1只羊的血清和淋巴结SFTSV核酸检测呈阳性,附着于该羊体表的1只长角血蜱病毒核酸也呈阳性,通过病毒分离获得2株SFTSV毒株,JS2012-yang01(羊)和JS2012-pi01(蜱)。序列分析结果显示,JS2012-yang01和JS2012-pi01的L、M和S基因片段的核苷酸同源性分别为99.8%、99.8%、100.0%,氨基酸同源性分别为99.6%、99.6%、100.0%,进化树分析显示两株病毒处于同一亚分支。2株毒株与中国其他地区流行的SFTSV毒株核苷酸序列同源性介于95.9%~99.6%(L基因),94.7%~99.5%(M基因)和95.0%~99.5%(S基因),与布尼亚病毒科白蛉病毒属其他毒株序列差异较大。结论该调查中分离自羊和蜱的SFTSV高度同源。蜱虫通过叮咬吸血,可能在SFTSV从动物到人的传播过程中起媒介作用。
Objective To investigate the prevalence of fever and thrombocytopenic syndrome (SFTSV) in sheep and tick ticks in a county in Jiangsu Province in the spring of 2012 and to analyze the whole genome sequence of the isolated strains. Methods SFTSV nucleic acid was detected by real-time fluorescence quantitative RT-PCR. All the positive samples were isolated and sequenced. The sequence was stitched and analyzed by Lasergene and MEGA4 software. Results SFTSV nucleic acid was detected in the serum and lymph node of seven sheep and one of the three H. pylori collected from the sheep, and one was also found in the body of the sheep Two SFTSV strains, JS2012-yang01 (sheep) and JS2012-pi01 (ticks) were obtained by virus isolation. Sequence analysis showed that the nucleotide homology of the L, M and S gene fragments of JS2012-yang01 and JS2012-pi01 were 99.8%, 99.8% and 100.0%, respectively. The amino acid homologies were 99.6% and 99.6% 100.0%. Phylogenetic tree analysis showed that the two viruses were on the same sub-branch. The homologies of the two strains to the SFTSV strains prevailing in other parts of China ranged from 95.9% to 99.6% (L gene), 94.7% to 99.5% (M gene) and 95.0% to 99.5% (S gene ), And Bunyaviridae other strains of the genus Pneumocystis strains are quite different. Conclusion SFTSV isolated from sheep and ticks in this survey was highly homologous. Ticks absorb blood through their bites and may play a mediating role in the transmission of SFTSV from animals to humans.