目的:探讨增强子结合蛋白(C/EBP)家族一个新基因HP8的人染色体定位.方法:按常规方法制备分裂期人淋巴细胞切片,用生物素标记HP8基因 3’-端 2.1kb cDNA作探针,进行荧光原位杂交(FISH).对FISH信号及染色体上DAPI结合条带同时拍照,二者重迭信号进行计数,结果:100个有丝分裂图中,有72个显示阳性信号位于同一对染色体,并且无其他非特异性杂交带,杂交效率约为70%.参照DAPI结合条带,阳性信号位于人19号染色体长臂13.1-13.2区带.结论:HP8基因定位于人19号染色体长臂 13.1-13.2区带. Objective: To investigate the human chromosome location of HP8, a new gene of the enhancer binding protein (C / EBP) family.Methods: The lymphocytes were dissected from human lymphocytes by routine methods and labeled with 3’-terminal 2.1kb cDNA of HP8 gene (FISH) .At the same time, FISH signal and DAPI binding band on the chromosome were photographed at the same time, and the overlapping signals were counted. As a result, 72 out of 100 mitosis showed that the positive signal was located on the same chromosome , And no other nonspecific hybridization bands, the hybridization efficiency was about 70% .Referring to the DAPI binding bands, the positive signals were located in the 13.1-13.2 region of the long arm of human chromosome 19. CONCLUSION: The HP8 gene is located on the long arm 13 of human chromosome 19 -13.2 zone.