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目的探讨化橘红提取物(extract of exocarpium citri grandis)对链脲佐菌素(STZ)诱导大鼠糖尿病心肌病的防治作用及其机制。方法 SD大鼠建立实验性2型糖尿病心肌病模型,随机分为模型对照组、化橘红高、中、低剂量治疗组、PPARγ激动剂吡格列酮治疗组(每组各15只),正常大鼠15只作为正常对照。观察不同剂量化橘红口服10、20、40mL/(kg·d)治疗对心肌结构功能损伤程度的影响;HE染色检测心肌组织结构改变,免疫印迹法检测心肌组织p 38 MAPK和cleaved caspase-3的表达,透射电镜观察心肌细胞超微结构。结果与正常对照组比较,模型组大鼠心脏组织心肌凋亡增多,HE染色可见心肌肥大、胶原沉积,心肌细胞超微结构损伤显著;化橘红干预组能减轻心肌凋亡、心肌肥大、胶原沉积等,同时能明显抑制p-p38 MAPK和cleaved caspase-3的表达。结论化橘红能防治糖尿病心肌病心肌结构功能损伤,其机制可能与抑制心肌p38 MAPK信号通路有关。
Objective To investigate the preventive and therapeutic effects of extract of exocarpium citri grandis on streptozotocin (STZ) -induced diabetic cardiomyopathy in rats. Methods SD rats were established experimental type 2 diabetic cardiomyopathy model and randomly divided into model control group, hypercholesterolaminemia, medium and low dose treatment group, PPARγ agonist pioglitazone treatment group (15 rats in each group), normal rats 15 Only as a normal control. To observe the effect of different doses of orange juice on myocardial structural dysfunction induced by 10, 20, 40mL / (kg · d) oral administration of myocardial ischemia. The changes of myocardial structure were observed by HE staining. The levels of p38 MAPK and cleaved caspase-3 The ultrastructures of cardiomyocytes were observed by transmission electron microscopy. Results Compared with the normal control group, the myocardial apoptosis in the model group was increased. The myocardial hypertrophy and collagen deposition were observed by HE staining. The ultrastructure damage of myocardial cells was significant. The intervention group could reduce the myocardial apoptosis, myocardial hypertrophy, collagen deposition , While significantly inhibiting the expression of p-p38 MAPK and cleaved caspase-3. Conclusion The orange juice can prevent myocardial structural dysfunction in diabetic cardiomyopathy, the mechanism may be related to inhibition of myocardial p38 MAPK signaling pathway.