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探讨在不同剂量农用混合稀土“常乐”的作用下,大鼠肝脏的形态、功能改变及其作用机理。以W istar大鼠为对象,用生理盐水和2.0、5.0、20.0m g.kg-1的常乐连续灌胃3个月后,采用生化方法检测了血清中天冬氨酸氨基转移酶(A ST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、γ-谷氨酰胺转移酶(GGT);应用常规组织学及透射电镜技术观察了大鼠肝脏光镜及电镜形态结构的变化;采用酶组织化学技术检测了肝细胞中琥珀酸脱氢酶(SDH ase)、葡萄糖-6-磷酸酶(G-6-Pase)及三磷酸腺苷酶(C a2+-ATPase)的变化。与对照组比较2.0、5.0m g.kg-1组大鼠肝脏结构、血清酶及SDH ase、G-6-Pase、C a2+-ATPase的活性均无明显改变;20.0m g.k-g 1组肝细胞索排列紊乱,汇管区有炎细胞浸润,糖原减少,线粒体嵴轻度肿胀,胆小管附近有较多电子密度高的致密体及溶酶体,SDH ase、G-6-Pase和C a2+-ATPase活性均明显降低。给予不同剂量的混合稀土,大鼠肝脏出现不同程度的改变,以20.0m g.kg-1组显著。
To investigate the morphological and functional changes of rat liver and its mechanism under different doses of agricultural rare earth “Changle”. Wistar rats as a target, with normal saline and 2.0, 5.0, 20.0m g.kg-1 of Changle continuous gavage 3 months after the use of biochemical methods to detect serum aspartate aminotransferase (A ST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (GGT). The morphology of the liver and liver were observed by conventional histology and transmission electron microscopy The changes of SDH ase, G-6-Pase and C a2 + -ATPase in hepatocytes were detected by enzyme histochemistry. Compared with the control group, the hepatic structure, serum enzymes and the activities of SDH ase, G-6-Pase and C a2 + -ATPase in 2.0 and 5.0 m g · kg-1 groups had no significant changes; Cell line disorder, the portal area with inflammatory cell infiltration, glycogen reduction, mitochondrial crista slightly swollen, more electron density near the small tube dense bodies and lysosomes, SDH ase, G-6-Pase and C a2 + -ATPase activity were significantly reduced. Different doses of mixed rare earths were given to rats, varying degrees of liver changes to 20.0m g.kg-1 group significantly.