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目的:研究蝎源活性肽对早期帕金森病(PD)大鼠脑源性营养因子(BDNF)及神经肽Y(NPY)的影响。方法:实验动物随机分为三组(n=11),分为早期PD模型组、假手术对照组和蝎源活性肽治疗组。将6羟多巴(6-OHDA,20μg/3μl含0.1%抗坏血酸生理盐水)注射到SD大鼠右侧纹状体制备早期PD模型,注射2周后,通过旋转行为学检测造模是否成功;造模成功的早期PD大鼠,和相应的对照组腹腔注射蝎源活性肽(2.20 mg/kg·d)处理1周。3周后,用免疫组织化学法检测大鼠脑源性营养因子及神经肽Y的免疫反应活性。结果:在6-OHDA给药侧,PD动物与假手术对照组相比较,BDNF免疫反应活性明显增强,NPY免疫反应活性明显减少,蝎源活性肽处理可以逆转这些异常改变。结论:改变PD早期中脑内NPY与BDNF的免疫反应活性是蝎源活性肽对早期PD大鼠中脑多巴胺能神经元的保护作用机制之一。
Objective: To study the effect of scorpion active peptides on brain derived neurotrophic factor (BDNF) and neuropeptide Y (NPY) in early stage of Parkinson’s disease (PD) rats. Methods: Experimental animals were randomly divided into three groups (n = 11), divided into early PD model group, sham operation control group and scorpion active peptide treatment group. Early 6-hydroxydopamine (6-OHDA, 20μg / 3μl saline containing 0.1% ascorbic acid) was injected into the right striatum of SD rats to prepare early PD model. After 2 weeks of injection, The successful PD model rats were injected intraperitoneally with scorpion active peptide (2.20 mg / kg · d) for 1 week. Three weeks later, the immunoreactivity of brain derived neurotrophic factor and neuropeptide Y was detected by immunohistochemistry. Results: Compared with the sham control group, the immunoreactivity of BDNF was significantly increased and the activity of NPY immunoreactivity was significantly decreased in the 6-OHDA administration group. The treatment with scorpion-derived active peptide could reverse these abnormal changes. Conclusion: It is one of the protective mechanisms of scorpion active peptides on the dopaminergic neurons in the midbrain of early PD rats that altering the immunoreactivity of NPY and BDNF in the early midbrain of PD.