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芦丁是黄酮类化合物之一,具有广泛的生理活性。本实验采用体外培养心肌细胞,研究了芦丁对体外培养心肌细胞缺氧、缺糠性损伤的保护作用。1 实验材料 芦丁由我校天然药物教研室提供,人参总皂甙由我校药理教研室提供,胶原酶为美国SIGMA产品,胰蛋白酶为美国DIFCO公司产品,小牛血清为长春生物制品厂产品,RPMI-1640培养液日本株式会社产品,WISTAR系大鼠、乳鼠由我校实验动物科提供,超氧化物歧化酶(SOD)、丙二醛(MDA)测试盒由南京建成
Rutin is one of the flavonoids and has a wide range of physiological activities. In this experiment, cardiomyocytes were cultured in vitro to study the protective effect of rutin against hypoxia and insufficiency of cultured cardiomyocytes in vitro. 1 Experimental materials Rutin is provided by the Natural Medicine Teaching and Research Office of our school. Ginseng total saponin is provided by the Department of Pharmacology, FDA. Collagenase is a US SIGMA product, trypsin is a product of the US DIFCO company, and calf serum is a product of Changchun Biological Products Factory. RPMI- 1640 Culture Solution Japan Products, Wistar Rats, and Suckling Rats are provided by the Laboratory Animal Division of our school, and superoxide dismutase (SOD) and malondialdehyde (MDA) test kits are built in Nanjing.