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目的 探讨幽门螺杆菌cagA(Hp cagA)菌株感染与胃癌组织中环氧化酶 Ⅱ (COX 2 )表达之间的关系 ,为胃癌的早期预防提供有价值的实验和理论依据。方法 采用流式细胞术定量检测及分析 31例胃癌组织及相应的癌旁正常胃组织中COX 2的表达水平 ,并通过PCR技术对胃癌组织中cagA基因进行扩增检测。结果 31例胃癌组织中 ,有 2 6例COX 2过表达 ,相应的 2 6例癌旁正常组织基本上无表达或弱表达。 18例cagA阳性的胃癌组织中COX 2的表达水平明显高于 13例cagA阴性的胃癌组织。结论 COX 2在胃癌组织中过度表达 ,cagA菌株感染可上调胃癌组织中COX 2的表达水平。可能存在cagA菌株感染之外的其他因素或机制调节COX 2的表达。因此 ,在根治Hp感染的同时 ,应用COX 2特异性抑制剂可能是一种有效预防胃癌发生、发展的新路
Objective To investigate the relationship between Hp cagA (Helicobacter pylori) infection and the expression of cyclooxygenase Ⅱ (COX - 2) in gastric cancer and to provide valuable experimental and theoretical evidences for the early prevention of gastric cancer. Methods Flow Cytometry was used to detect and analyze the expression of COX-2 in 31 cases of gastric carcinoma and its adjacent normal gastric tissues. The cagA gene in gastric cancer tissues was amplified by PCR. Results Twenty-six cases of gastric cancer tissues were overexpressed by COX 2, and the corresponding 26 normal tissues were almost no expression or weakly expressed. 18 cases of cagA positive gastric cancer COX 2 expression was significantly higher than 13 cases of cagA negative gastric cancer. Conclusion COX 2 overexpression in gastric cancer tissue, cagA infection can up-regulate the expression of COX 2 in gastric cancer. There may be other factors or mechanisms outside the cagA strain that regulate the expression of COX2. Therefore, in the cure of Hp infection, the application of COX 2-specific inhibitors may be a new way to effectively prevent the occurrence and development of gastric cancer