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两步免疫组织化学染色是以辣根过氧化物酶 (ＨＲＰ)标记的多聚体与抗鼠或兔ＬｇＧ的二抗相连 ,操作时只需进行一抗和二抗两步孵育。由于多聚物提供了增强的信号扩大系统 ,使其比ＳＰ法免疫组织化学染色具有更高的灵敏性 ;多聚物中无生物素 ,不会与组织中生物素结合而造成非特异
Two-step immunohistochemical staining is a horseradish peroxidase (HRP) labeled multimers and anti-mouse or rabbit LgG secondary antibody connected to the operation only need to conduct a two-step primary and secondary antibody incubation. Because polymers provide an enhanced signal amplification system that is more sensitive than the SP immunohistochemical staining, there is no biotin in the polymer and it does not bind to biotin in the tissue causing non-specific