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目的观察二烯丙基三硫化物(diallyl trisulfide,DATS)对离体肾内动脉张力的影响及其机制。方法 SD大鼠体质量250～300 g,脱臼处死后,显微操作下取出肾内动脉,制备成1.8～2.0 mm长的血管条,每根血管条固定于微血管测定仪的浴槽内,记录张力变化。分别给予血管收缩剂苯肾上腺素(Phe)、血栓素A2受体激动剂(U46619)、5-羟色胺(5-HT)和KCl刺激血管产生持续性收缩,采用累积给药法加入DATS,观察不同浓度的DATS对肾内动脉张力的影响。观察DATS舒张效应的同时用等体积的药物溶剂二甲基亚砜(DMSO)作为对照。结果 DATS能呈浓度依赖性诱导舒张1μmol.L-1 Phe、0.1μmol.L-1 U46619和2μmol.L-1 5-HT预收缩内皮完整的肾内动脉环,pD2分别为(6.06±0.17),(6.14±0.26)和(5.37±0.16),其最大舒张率(Emax)分别为(91.24±2.71)%,(93.44±2.14)%和(92.51±1.15)%;但是,对60 mmol.L-1 KCl预收缩的肾内动脉环无明显舒张作用;分别用eNOS抑制剂L-NAME和sGC抑制剂ODQ预处理内皮完整的肾内动脉环不影响DATS舒张效应;去除肾内动脉血管内皮也不影响DATS对其舒张作用。结论 DATS有浓度依赖性舒张大鼠肾内动脉作用,无明显内皮依赖性。
Objective To observe the effect of diallyl trisulfide (DATS) on tension of isolated renal artery and its mechanism. Methods The body weight of SD rats was 250-300 g. After dislocation and death, the renal arteries were removed under micromanipulation to prepare vascular strips of 1.8-2.0 mm in length. Each vascular strip was fixed in the bath of the microvascular analyzer and the tension was recorded Variety. The vasoconstrictor phenylephrine (Phe), thromboxane A2 receptor agonist (U46619), 5-hydroxytryptamine (5-HT) and KCl were given to stimulate the blood vessels to produce sustained contraction, and the cumulative administration method was used to add DATS to observe the difference Effect of DATS Concentration on Renal Artery Tension. Observe the diastolic effect of DATS while using an equal volume of drug solvent dimethyl sulfoxide (DMSO) as a control. RESULTS: DATS preconditioned intact renal artery rings in a dose-dependent manner induced relaxation of 1 μmol.L-1 Phe, 0.1 μmol.L-1 U46619 and 2 μmol.L-1 5-HT, respectively (p = 6.06 ± 0.17) , (6.14 ± 0.26) and (5.37 ± 0.16), respectively. The maximal relaxation rates were (91.24 ± 2.71)%, (93.44 ± 2.14)% and (92.51 ± 1.15)%, respectively. -1 KCl precontracted renal artery ring no significant relaxation; respectively, with eNOS inhibitor L-NAME and sGC inhibitor ODQ intact endothelium within the renal artery does not affect DATS diastolic effect; removal of renal artery endothelium also Does not affect DATS its relaxation effect. Conclusion DATS plays a role in renal artery in a concentration-dependent manner in rats with no obvious endothelium-dependent.