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目的 探讨IL 2 4基因对C6大鼠胶质瘤细胞生长状况的影响。方法 应用逆转录病毒载体 ,将IL 2 4基因导入C6细胞 ,经G4 18筛选后获得表达IL 2 4分子的阳性细胞克隆C6 /IL 2 4 ;用RT PCR方法检测目的基因表达 ;四甲基偶氮唑蓝 (MTT)法检测细胞体外增殖状况 ,流式细胞技术检测细胞的增殖活性 ,并制作荷瘤动物模型 ,观察C6 /IL 2 4和C6细胞的体内致瘤性。结果 RT PCR检测表明 ,外源IL 2 4基因于mRNA水平在C6 /IL 2 4细胞已获得稳定表达。C6 /IL 2 4细胞系的体外增殖性较亲代C6细胞明显下降 ,流式细胞术检测其细胞增殖指数 (PI)为 (2 9.71± 0 .89) %。 9只接种C6 /IL 2 4细胞的实验组大鼠中 ,6只颅内成瘤 ,肿瘤体积为 (14 .0 8± 9.81)mm3 ,明显小于接种C6细胞大鼠的肿瘤体积 (P <0 .0 5 )。结论 外源性IL 2 4基因可部分抑制胶质瘤细胞异常增殖的肿瘤特性。
Objective To investigate the effect of IL 2 4 gene on the growth of C6 glioma cells. Methods IL2 4 gene was introduced into C6 cells by retroviral vector and positive cells clone C6 / IL2 4 expressing IL2 4 was screened by G418. The expression of target gene was detected by RT PCR. MTT assay was used to detect the proliferation of cells in vitro. Flow cytometry was used to detect the proliferative activity of the cells. Animal models of tumor were established. The in vivo tumorigenicity of C6 / IL 2 4 and C6 cells was observed. Results RT-PCR showed that exogenous IL 2 4 gene was stably expressed in C6 / IL 2 4 cells at the mRNA level. The proliferation of C6 / IL 2 4 cell line was significantly lower than that of the parental C6 cells, and the cell proliferation index (PI) by flow cytometry was (2 9.71 ± 0.89)%. Among the 9 experimental C6 / IL 2 4-inoculated groups, 6 had intracranial tumor formation with a tumor volume of (14.0 ± 9.81) mm 3, which was significantly smaller than that of the C6 group .0 5). Conclusion Exogenous IL 2 4 gene can partially inhibit the tumor characteristics of abnormal proliferation of glioma cells.