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目的观察了解头低位卧床期间人体肠道益生菌双歧杆菌属、乳酸杆菌属的数量及多样性的变化趋势。方法采集受试者-6°头低位连续卧床30d期间肠道便样,通过平板可培养技术、浓度梯度凝胶电泳技术和Ion Torrent基因组测序技术平台等实验方法,检测分析肠道粪便益生菌。结果卧床期间,可培养肠道菌群中的双歧杆菌、乳杆菌数量显著减少,益生菌中双歧杆菌出现不规律的缺失与恢复,肠道菌群多样性指数下降。结论头低位卧床期间肠道益生菌多样性发生了改变。益生菌数量减少,多样性指数下降,头低位卧床对肠道菌群有不利的影响。
Objective To investigate the changes of the number and diversity of Bifidobacterium and Lactobacillus in human intestinal probiotics during the first bed rest. Methods Intestinal stools were collected during the first 30 days of continuous -6 ° head-down bed rest. The probiotics in intestinal faeces were detected by plate culturable technique, concentration gradient gel electrophoresis and Ion Torrent genome sequencing technology. Results During bedridden, the number of bifidobacteria and lactobacilli in the culturable intestinal flora was significantly reduced, the bifidobacteria in probiotics were irregularly deleted and recovered, and the diversity index of intestinal flora was decreased. Conclusion The diversity of intestinal probiotics in the head-lying bedridden period has changed. The number of probiotics decreased, the diversity index decreased, and the head-lodged bed had an adverse effect on intestinal flora.