Construction of Hybrid Enzyme from HEC-SOD and Cu,Zn-SOD

来源 :高等学校化学研究(英文版) | 被引量 : 0次 | 上传用户:nola0724
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Human extracellular superoxide dismutase(hEC-SOD) is a secreted tetrameric protein involved in the protection of a human body from oxygen free radicals. Its three-dimensional structure has not been confirmed. hEC-SOD couldn′t be expressed in E.coli. We constructed a hybrid enzyme, which comprises the N-terminal and C-terminal domains from hEC-SOD, fused it to human Cu,Zn-SOD. The hybrid enzyme is expressed successfully in E.coli. Further, we analyzed the expression of hEC-SOD in E.coli by mRNA differential displaying.
其他文献
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7
Applying the generalized method, which is a direct and unified algebraic method for constructing multiple travelling wave solutions of nonlinear partial differe
A new benzoazacrown ether fluorescence sensor was synthesized with 9-anthrylmethyl chloride and benzoaza-15-crown-5 in CH3CN, which particularly shows a strong
The title complex (enH2){NH4[Co(en)3][Mo2O7(C2O4)]}2·2H2O (C18H70Co2Mo4- N16O24, Mr = 1396.52) was obtained under hydrothermal conditions and its crystal struc
ONIOM quantum mechanics method is used in this paper to study one-carbon unit transfer from imidazolidine to 6-aminouracil derivates. The computation results sh
C60-Containing poly(ethylene oxide) (PEO) was synthesized by a new method. Molecular structural characterization for the polymers was presented by 1H-NMR, infra
Keggin anions behave differently from each other when they react with nucleic acids. The molybdenum series exhibits oxidative cleavage activity towards AMP and
We introduce a first-principles density-functional theory, i.e. the finite-difference pseudopotential densityfunctional theory in real space and the Langevin mo
This paper deals with the problem of uniqueness of meromorphic functions,and gets the following result: There exists a set S with 13 elements such that any two
We investigated the neutral Higgs boson pair production at the CERN Large Hadron Collider (LHC) in the SM with four families. We found that the gluon-gluon fusi