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目的:探讨脓毒症小鼠心肌损伤与中性粒细胞浸润的关系。方法:复制脓毒症动物模型,分对照组、假手术组、造模组,并设不同的时间点(2h、4h、8h、12h)。采用双抗夹心酶联免疫法(ELISA)检测血清肌钙蛋白(IcTnI),并测心肌组织髓过氧化物酶(MPO)活性。结果:正常组与假手术组各时间点血清cTnI、心肌组织MPO活性均无显著性差异。造模组心肌组织MPO活性较正常组和假手术组均有明显升高(P<0.05),且随着时间进展而增加;造模组血清cTnI浓度随着时间进展而增加,2小时与正常组及假手术组无显著性差异(P>0.05),4小时后显著高于正常组和假手术组(P<0.05);血清cTnI浓度与心肌组织MPO活性呈显著正相关(r=0.700,P=0.000)。结论:脓毒症心肌损伤时,心肌组织存在中性粒细胞浸润,中性粒细胞浸润程度与心肌损伤显著正相关。
Objective: To investigate the relationship between myocardial injury and neutrophil infiltration in septic mice. Methods: The animal model of sepsis was divided into control group, sham operation group and model group, with different time points (2h, 4h, 8h, 12h). Serum troponin (IcTnI) was detected by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA), and myocardial myeloperoxidase (MPO) activity was measured. Results: There was no significant difference of cTnI in serum and MPO in myocardium between normal group and sham operation group at each time point. Compared with the normal group and the sham operation group, MPO activity of myocardial tissue in model group was significantly increased (P <0.05), and increased with time; the concentration of cTnI in model group increased with time, (P> 0.05). After 4 hours, the level of cTnI in myocardium was significantly positively correlated with MPO activity (r = 0.700, P < P = 0.000). CONCLUSION: Myocardial tissue with neutrophil infiltration and neutrophil infiltration is significantly and positively correlated with myocardial injury in septic myocardial injury.