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目的探讨舒肝颗粒对抑郁模型大鼠海马神经元凋亡、脑组织caspase-3蛋白及外周血中细胞因子水平的影响。方法将40只雄性SD大鼠随机分为对照组、模型组、舒肝组和氟西汀组,每组10只,慢性轻度不可预见性应激和孤养方式制备抑郁模型。结果 21 d后模型组、舒肝组和氟西汀组行为学指标明显低于对照组,而舒肝组和氟西汀组明显高于对照组,差异有统计学意义(P<0.05)。模型组、舒肝组和氟西汀组海马神经元凋亡数和脑组织caspase-3蛋白表达明显高于对照组,而舒肝组和氟西汀组低于对照组,差异有统计学意义(P<0.05)。模型组、舒肝组和氟西汀组外周血TNF-α、IL-2和IL-8水平明显高于对照组,而舒肝组和氟西汀组低于对照组,差异有统计学意义(P<0.05)。结论舒肝颗粒可改善抑郁模型大鼠的行为学,修复神经元细胞损伤,降低脑组织caspase-3蛋白和外周血中细胞因子表达。
Objective To investigate the effect of Shugan Granule on apoptosis of hippocampal neurons, caspase-3 protein in brain tissue and cytokines in peripheral blood of depression model rats. Methods Forty male Sprague-Dawley rats were randomly divided into control group, model group, Shugan group and fluoxetine group, with 10 in each group. Chronic mild unpredictable stress and loneliness were used to prepare depression model. Results After 21 days, the behavioral indexes in model group, Shugan group and fluoxetine group were significantly lower than those in control group, while those in Shugan group and fluoxetine group were significantly higher than those in control group (P <0.05). The number of apoptotic hippocampal neurons and the expression of caspase-3 protein in model group, Shugan group and fluoxetine group were significantly higher than those in control group, while those in Shugan group and fluoxetine group were lower than those in control group (P <0.05). The levels of TNF-α, IL-2 and IL-8 in model group, Shugan group and fluoxetine group were significantly higher than those in control group, while those in Shugan group and fluoxetine group were lower than those in control group (P <0.05). Conclusion Shugan granules can improve the behavior of depression model rats, repair neuronal cell injury, reduce the expression of caspase-3 protein in brain tissue and the expression of cytokines in peripheral blood.