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用离子交换层析和亲和层析方法,从人新鲜胎盘中分离提纯血栓调节蛋白,并用发色底物S_(2366)检测法、SDS-PAGE电泳、等电聚集和氨基酸成分分析鉴定其抗原性和纯度。对该蛋白的功能特性及意义进行了初步探讨。
Thrombomodulin was isolated and purified from human fresh placenta by ion exchange chromatography and affinity chromatography, and its antigenicity was identified by S 2366 assay, SDS-PAGE electrophoresis, isoelectric focusing and amino acid composition analysis And purity. The functional properties of the protein and the significance of a preliminary study.