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1995年以来我国造血干细胞工程与相关的生物学领域的研究发展迅速。有关造血干 祖细胞基因表达的研究 ,上海国家人类基因组研究中心陈竺、陈赛娟等为正常和急性白血病人骨髓造血干祖细胞cDNA文库的基因表达建立了一套先进的工作体系。他们在许多白血病细胞系的干 祖细胞中发现了 30 0个新的相关基因。中山大学医学院李树浓、黄绍良等从人的桑葚期胚胎干细胞成功地诱导出造血细胞等。北京输血研究所裴雪涛等从成人和胎儿的骨髓分离出成年源干细胞 ,又进一步诱导分化为骨、软骨、脂肪和神经原细胞等。他们成功地构建了胎儿和成人间充质干细胞cDNA扣除文库 ,获得了胎儿和成人间充质干细胞的差异表达基因及在胎儿特异表达基因。中国医学科学院天津血液学研究所、国家血液学重点实验室赵春华等证实从胚胎胰腺、骨髓和肝脏中都可以分离出人间充质干细胞 ,又证明G CSF可以使输注的间充质干细胞在体内促造血重建。北京基础医学研究所毛宁等的实验不支持间充质干细胞可以“横向分化”。最近他们发现小鼠胚胎干细胞的体外分化重现了胚胎早期造血发生的生物学程序以及Smad5基因调控在胚胎造血发生中的必要性和多样性 ,又表明其上游配体TGF beta家族分子在胚胎发生中的作用和特点。本文针对干细胞可塑性研究作了评?
Since 1995, the research in the field of hematopoietic stem cell engineering and related biology has been developing rapidly. On the hematopoietic stem and progenitor cell gene expression research, Shanghai National Human Genome Research Center Chen Zhu, Chen Sai-Juan and other normal and acute leukemia bone marrow hematopoietic stem and progenitor cells cDNA library gene expression established an advanced working system. They found 30 0 new related genes in stem progenitor cells of many leukemia cell lines. Sun Yat-sen Medical School Li Shu-rong, Huang Shaoliang, etc. from human mulberry embryonic stem cells successfully induced hematopoietic cells. Pei Xuetao Beijing Institute of Blood Transfusion separated adult and fetal bone marrow from adult source stem cells, and further induced differentiation into bone, cartilage, fat and neuronal cells. They successfully constructed cDNA subtractive libraries of fetal and adult mesenchymal stem cells and obtained differentially expressed genes in fetal and adult mesenchymal stem cells and genes specifically expressed in the fetus. Chinese Academy of Medical Sciences, Tianjin Institute of Hematology, National Institute of Hematology, Zhao Chunhua and other confirmed from the embryonic pancreas, bone marrow and liver can be isolated from human mesenchymal stem cells, and G CSF can make infusion of mesenchymal stem cells in vivo Promote blood reconstruction. Experiments by Mao Ning, a Beijing Institute of Basic Medical Research, do not support “mesenchymal stem cells that can differentiate laterally.” Recently, they found that in vitro differentiation of mouse embryonic stem cells reproduces the biological processes of embryonic hematopoiesis and the necessity and diversity of Smad5 gene regulation in embryonic hematopoiesis, and also shows that the upstream ligand TGF beta family of molecules in embryogenesis In the role and characteristics. This article for the evaluation of stem cell plasticity made?