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为观察复方萘酚喹及其各单药 (磷酸萘酚哇、双氢青蒿素、甲氧苄啶 )对伯氏疟原虫ANKA株红内期超微结构的影响 ,将感染伯氏疟原虫ANKA株的小鼠分别以复方萘酚喹 2 6 0mg·kg- 1·d- 1× 1d (内含磷酸萘酚喹 149mg、双氢青蒿素 37mg、甲氧苄啶 74mg) ,磷酸萘酚喹 149mg·kg- 1·d- 1× 1d ,双氢青蒿素 180mg·kg- 1·d- 1× 1d和甲氧苄啶 1152mg·kg- 1·d- 1× 1d灌胃给药。给药后 1、 2、 4、 8、 12和 2 4h ,采血作标本 ,经包埋 ,切片 ,染色后 ,用电镜镜检。结果 :复方萘酚喹于给药 2h后多数晚期滋养体出现质膜肿胀、破裂 ,有的呈空腔状 ;食物泡膜肿胀 ;线粒体膜肿胀、分层、剥离 ;色素颗粒变形。给药 4h后进一步加剧。 8h后小部分晚期滋养体结构已经融化崩解 ,内容物结构模糊。 12h后绝大多数晚期滋养体结构已完全破坏或消失。结论 :复方萘酚喹对伯氏疟原虫红内期超微结构的影响较其组分中各单药起效更快、力度更强和作用相对彻底。
To observe the effect of compound naphthoquine and its single agents (Naphthol Phosphate, Dihydroartemisinin and Trimethoprim) on the ultrastructure of the ANKA strain of Plasmodium berghei during the red stage, the Plasmodium berghei ANKA strain mice were treated with compound naphthoquine 260 mg · kg-1 · d-1 × 1d (containing 149 mg of phosphinophenol, 37 mg of dihydroartemisinin and 74 mg of trimethoprim) Quinoline 149 mg · kg -1 · d -1 × 1d, dihydroartemisinin 180 mg · kg -1 · d -1 × 1d and trimethoprim 1152 mg · kg -1 · d -1 × 1d. Blood samples were collected at 1, 2, 4, 8, 12 and 24 h after drug administration, embedded, sectioned, stained and examined by electron microscopy. Results: Naphthol quinine showed swelling and rupture of plasma membrane in most of late trophozoites after 2h administration. Some of them showed cavity-like shape; swelling of food bubble membrane; swelling, delamination and delamination of mitochondria membrane; deformation of pigment granules. 4h after administration further exacerbated. After 8h, a small part of late trophozoite structure has melted and collapsed, and the content structure is fuzzy. After 12h the vast majority of late trophozoites have completely destroyed or disappeared. CONCLUSION: The effect of compound naphthoquine on the ultrastructure of Plasmodium berghei during the red stage is faster, stronger and more complete than that of the single drug.