本文为探讨注射器组装后在脉动真空灭菌柜灭菌的效果，使用了嗜热脂肪芽胞杆菌菌片及染菌法进行了监测。结果表明，注射器组装后在脉动真空灭菌柜灭菌达到了无菌要求。组装后与分开包装灭菌无差异，为供应室在今后的工作中使用此法提供了理论依据 In this paper, we explored the effect of sterilizing the pulsating vacuum sterilizer after the syringe was assembled, and then used the Bacillus stearothermophilus and the bacterial infection method to monitor the bacteria. The results showed that sterility of the pulsation vacuum sterilizer reached a sterility requirement after the syringe was assembled. After assembly, there is no difference between separate packaging and sterilization, which provides a theoretical basis for the supply room to use this method in future work