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产毒性大肠杆菌(ETEC)是发展中国家儿童和幼畜腹泻的主要病原体。为了解了我市儿童感染ETEC的情况,我们于1995～1998年对腹泻病监测点5岁以下儿童进行了ETEC检测,结果报告如下。1 材料和方法1.1 标本来源 选择交通便利一乡镇2个自然村作为腹泻病监测点,由监测点医生对腹泻儿童在未服药前采便,接种C—B运送培养基,当日送我站细菌室进行分离培养。1.2 ETEC分离 标本直接划种EMB曙红—美蓝琼脂,37℃24h培养,每个平板上挑取3～5个典型大肠杆菌菌落,用半固体
Toxicogenic E. coli (ETEC) is a major causative agent of diarrhea in children and young animals in developing countries. To understand the situation of children infected with ETEC in our city, we conducted ETEC tests on children under 5 years of age at the monitoring point of diarrhea from 1995 to 1998. The results are reported as follows. 1 Materials and methods 1.1 Selection of source of specimens Convenient transportation A village of 2 villages as a monitoring point for diarrhea by the monitoring point of the children of diarrhea in children before taking the extract will not be vaccinated, C-B transport medium, the day sent to my station bacteria room Isolation and culture. 1.2 ETEC isolated specimens directly planted EMB Eosin - methylene blue agar, 37 ℃ 24h culture, each plate to pick 3 to 5 typical E. coli colonies, with a semi-solid