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利用病毒诱导的基因沉默(VIGS)机制可以从基因组序列入手来进行植物基因功能研究,这是一种反向遗传学方法.本室曾成功利用中国番茄黄化曲叶病毒(TYLCCNV)的卫星DNA构建了基因沉默的载体.本研究对该载体进行了改良,在本氏烟上对Su基因的沉默测试表明,改良后的载体与原载体诱导沉默的效率没有差别,但是改良后的载体构建沉默克隆的过程得以简化.进一步用改良后的沉默载体分别抑制矮牵牛内源基因Su和CHS基因的表达.含Su基因的沉默载体接种矮牵牛大约2周后,矮牵牛沿叶脉开始黄化;含CHS基因的沉默载体接种矮牵牛约1个月后,新开的牵牛花上出现花色的褪变,并由单色花变成了杂色花.
Using the virus-induced gene silencing (VIGS) mechanism to study plant gene function from genomic sequences, a reverse genetics approach, we successfully used satellite DNA from TYLCCNV A gene silencing vector was constructed.The vector was modified in this study, and silencing Su gene in N. benthamiana showed that there was no difference in efficiency between the modified vector and the original vector in inducing silencing, but the improved vector construction silence The process of cloning was simplified, and the expression of Su and CHS genes in petunia were further inhibited by the modified silencing vectors, respectively. About 2 weeks after inoculation with the silencing vector containing Su gene, ; About 1 month after inoculation of petunia with the CHS gene-silencing vector, the newly opened morning glory appeared faded and changed from monochromatic flower to variegated flower.