论文部分内容阅读
目的 观察电刺激治疗对大鼠脑梗死后运动功能和脑星形胶质细胞活性的影响 ,方法 易卒中型肾血管性高血压大鼠 (RHRSP)右侧大脑中动脉闭塞后 ,电刺激其瘫痪肢体的 4个穴位 ,以走横木试验 (BWT)评价大鼠的精细运动功能恢复情况 ,免疫组化法观察脑星形胶质细胞活性。结果 经电刺激治疗的大鼠 ,运动功能的恢复较对照组明显改善 (P<0 0 1) ;电刺激治疗组在坏死边缘区 (A区 )和远隔区 (B)星形胶质纤维酸性蛋白 (GFAP)表达均较对照组高 ;GFAP阳性细胞浆平均光密度值变化情况与GFAP表达变化基本相同。结论 电刺激可通过增强星形胶质细胞活性而促进瘫痪肢体功能恢复。脑梗死后星形胶质细胞在其修复中起了重要的作用。
Objective To observe the effects of electrical stimulation on motor function and brain astrocyte activity after cerebral infarction in rats. Methods After right middle cerebral artery occlusion in rats with stroke-prone renovascular hypertension (RHRSP), electrical stimulation of paralysis Four acupoints of limbs were taken to evaluate the recovery of fine motor function in BWT rats. The activity of astrocytes was observed by immunohistochemistry. Results After electrical stimulation, the recovery of motor function was significantly improved in the control group (P <0.01). The electrical stimulation of the astrocytes in the marginal zone (A) and distal zone (B) of the astrocytes The expression of GFAP was higher than that of the control group. The change of average optical density of GFAP-positive cytoplasm was almost the same as that of GFAP. Conclusion Electrical stimulation can promote functional recovery of paralyzed limbs by enhancing the activity of astrocytes. Astrocytes play an important role in the repair of cerebral infarction.