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目的:观察定心方对ApoE基因敲除小鼠(ApoE-/-小鼠)主动脉粥样硬化病变的影响,并初步探讨血清内脏脂肪素(visfatin)在其中的作用。方法:将40只8周龄ApoE-/-小鼠随机分为5组。模型组灌服等剂量生理盐水;定心方低、中、高剂量组灌服定心方药液9.295g/(kg.d)、18.59g/(kg.d)、37.18g/(kg.d);辛伐他汀组灌服辛伐他汀5mg/(kg.d)。另设相同基因背景和周龄的C57BL/6J小鼠为空白对照组。12周后测定小鼠体质量、血清生化指标及visfatin、hs-CRP、TNF-a水平;对主动脉粥样硬化病变进行病理学检查。结果:与模型组相比,定心方低、中、高剂量组小鼠体重增加值明显降低(P<0.05),定心方高剂量组血脂水平及血清visfatin、hs-CRP、TNF-α含量显著低于模型组(P<0.01)。定心方低、中、高组斑块面积分别较模型组减少39.3%、75.0%、86.5%(P<0.01)。结论:定心方可降低ApoE-/-小鼠血脂水平,抑制动脉粥样硬化的形成,其作用机制可能与降低血清visfatin水平有关。
Objective: To observe the effect of Dingxin Recipe on aortic atherosclerosis in ApoE knockout mice (ApoE - / - mice) and to explore the role of visfatin in it. Methods: Forty eight-week old ApoE - / - mice were randomly divided into five groups. The model group was fed with the same dose of normal saline. The low, medium and high dose group was given 9.295g / (kg · d), 18.59g / (kg.d), 37.18g / (kg. The simvastatin group was given simvastatin 5 mg / (kg.d). Another C57BL / 6J mice with the same genetic background and age were used as blank control group. After 12 weeks, the body weight, serum biochemical indexes and the levels of visfatin, hs-CRP and TNF-a in mice were determined. Pathological changes of aortic atherosclerosis were observed. Results: Compared with the model group, the body weight gain of the low, medium and high dose group was significantly lower (P <0.05), and the level of serum lipid, serum visfatin, hs-CRP and TNF-α Content was significantly lower than the model group (P <0.01). The plaque area of low, medium and high groups were reduced by 39.3%, 75.0% and 86.5% respectively (P <0.01) compared with model group. Conclusion: Dingxin Recipe can reduce the blood lipid level and inhibit the formation of atherosclerosis in ApoE - / - mice, which may be related to the decrease of serum visfatin level.