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潜根线虫(Hirschmanniella spp.)是一类寄生于水稻根部的重要病原线虫,在我国多个省份和地区皆有分布,然而江苏稻区有关该线虫发生和危害鲜有报道。从江苏省农科院水稻实验田采集的水稻根部分离到大量潜根线虫,利用光学和扫描电镜显微观察确定所分离到的线虫优势种群为细尖潜根线虫(Hirschmanniella mucronata),de-Man形态学数据显示江苏分离群体与其他已报道的H.mucronata分离群体具有一定差异,但基本处于标准模式值范围。分别对ITS-rRNA、28S rRNA D2-D3扩展区、18S rRNA、细胞色素氧化酶c亚基I(COI)和热激蛋白90(Hsp90)序列进行PCR扩增,新获得的H.mucronata ITS序列与中国台湾和比利时分离群体具有高度相似性,同时比较各分离群体的ITS1、5.8S和ITS2序列碱基变异,显示我国江苏、台湾和比利时群体差异最小。基于28S rRNA D2-D3扩展区、18S rRNA和ITS-rRNA序列构建的贝叶斯和最大似然进化树将潜根线虫划分为3个进化分支,与已报道的其他H.mucronata种群共同位于第二分支。
Hirschmanniella spp. Is a kind of important pathogenic nematode parasitized in the root of rice. It has been distributed in many provinces and regions in China. However, the occurrence and damage of nematode in Jiangsu rice area are rarely reported. A large number of root-knot nematodes were isolated from the rice roots collected from the experimental field of Jiangsu Provincial Academy of Agricultural Sciences, and the predominant nematode populations were identified as Hirschmanniella mucronata and de-Man by optical and scanning electron microscopy. Morphological data show that there are some differences between the isolated populations of Jiangsu and other reported H. mucronata populations, but they are basically in the range of standard mode values. The sequences of ITS-rRNA, 28S rRNA D2-D3, 18S rRNA, cytochrome oxidase subunit I (COI) and heat shock protein 90 (Hsp90) were amplified by PCR. It has a high degree of similarity with the populations isolated from Taiwan and Belgium in China. Comparing the nucleotide variation of ITS1, 5.8S and ITS2 sequences among different populations showed that there was the least difference between Jiangsu, Taiwan and Belgium in our country. Based on the 28S rRNA D2-D3 extension region, 18S rRNA and ITS-rRNA sequences were constructed by Bayesian and Maximum Likelihood evolutionary trees, which were divided into three evolutionary branches, which are closely related to other reported H. mucronata populations Two branches.